3D Microscopy

3D MICROSCOPY usually involves taking 2 images from slightly different angles, using a tilting stage. For 3D the main difficulty is obtaining adequate depth of field. Even with low power objectives a single shot's DOF is only a few microns. A now-popular method around this problem is called "Image Stacking" or "Optical Slicing". In this, a stepper motor focus drive, controlled by a computer, is used to rack the focus across the sample in small steps. The digital images are collected and processed using a program that combines all the in-focus bits from each "slice" to produce a composite expanded-depth-of-field image. Our subjects includes chemicals, fabrics, plants, and a wide range of other materials and living (or once living) objects. 

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Focus drive used to Z-scan a fiber tangle (for example)